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KMID : 0380919960250010150
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Journal of the Korean Society of Food Nutrition
1996 Volume.25 No. 1 p.150 ~ p.156
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Characteristics of a Interspecific Protoplast Fusant from Lactobacillus acidophilus 88 and Lactobacillus bulgaricus IFO 13953
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Jo Young-Bae
Kim Hye-Jeong
Kim Sung-Koo
Baik Hyung-Suk
Jun Hong-Ki
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Abstract
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An interspecific fusant was made from the protoplasts of two strains of Lactobacillus genus(streptomycin and lincomycin resistant L. bulgaricus and kanamycin resistant L. acidophilus 88). The functional properties of the fusant were examined by determining bacteriocin productivity, acid-producing activity, ability of carbohydrates fermentation and three important enzyme activities. The recombinant strain revealed bacteriocin productivity. Acid production and β-galactosidase, phospho-β-galactosidase, lipase and protease activity of L. bulgaricus were better than those of L. acidophilus 88. Among fusants, β-galactosidase activity of two strains were better than that of the parent strains but phospho-β-galactosidase activity remarkably lower. One fusant revealed the improved proteolysis compared to that of the parent strains. Lipase activity of L. bulgaricus was better than that of L. acidophilus but another fusant exhibited the highest lipase activity.
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KEYWORD
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Lactobacillus acidiophilus, Lactobacillus bulgaricus, electrofusion, reconbinant strain
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